The global awards, now in their 14th year, celebrate the contribution colleagues make to their local communities - whether through fundraising or volunteering, mentoring, one-off activities or an ongoing commitment.
The lively event, which was held at the historic El Antiguo Convento, was hosted by Marcus Agius and attended by a 250-strong audience of colleagues, community managers and senior leaders from across the business.
A total of 376 nominations from 27 countries, covering every part of the business, were received this year.
Sol Moreno de los Rios, a Barclays Capital colleague from Spain, was named Overall Winner for her commitment to improving the social integration of young people affected by Down's syndrome, which has benefited more than 120 children and their families.
Team Bhurjwadi from Barclays Corporate, India, also received a double honour, scooping both the Team Achievement of the Year - Asia Pacific accolade, and a Special Award after impressing judges with the scope and impact of their project.
More than 400 employees joined forces to provide 160 families in Bhurjwadi, a remote region about 100km from Mumbai, with water-efficient toilets.
In addition to the Overall Winner and Special Award, ten category winners were announced from a shortlist of 30 finalists.
Each category winner receives £2,500 to donate to the charity of their choice.
The Overall Winner will be offered an international volunteering opportunity at a Barclays Global Community Investment project.
Marcus said: "Every year, the quality of the nominations increases which makes selecting the finalists, and then the category winners, ever more difficult. We've had such a diverse range of entries this year from incredible fundraising activities, such as LifeCycle and Chris Rowe's Dribblethon, to programmes where colleagues give their time and skills to help disadvantaged people into employment, such as the Visit Prison team and the Java IT team.
"All are unique. All have had a lasting impact. And all are deserving of an award."
He added: "Congratulations to them - and indeed to all of our nominees. But the real winners tonight are the people you and your colleagues have helped and supported in communities across the world. On their behalf, a big thank you to you all."
The ceremony was also attended by Group Chief Executive John Varley, who set the awards in the wider context of Barclays Responsible Banking strategy, telling guests: "Community investment plays a pivotal role in the success of any business. For banking, though, that role is even more important.
"Our success depends on the success of the communities in which we live and work."
And he continued: "We're here tonight to celebrate some great things Barclays colleagues have achieved outside the workplace.
"These achievements are not separate from what we achieve commercially. Far from it. The activities we are celebrating tonight are integral to our ability to ensure our stakeholders see us as a responsible bank."